K /H antiporter for K efflux

K⁺/H⁺ antiporter for K⁺ efflux

BSU09860

BSU_09860

495

498

K /H antiporter for K efflux

K⁺/H⁺ antiporter for K⁺ efflux

BSU09860

BSU_09860

1,060,427 -> 1,060,924

1,060,427 → 1,060,924

contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:RCK+C-terminal+domain* x])

contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score x])

cell membrane (peripheral) (according to Swiss-Prot)

cell membrane (peripheral) (according to UniProt)

MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]

GP2078 (''[[gene|khtS]]-[[gene|khtT]]-[[gene|khtU]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]

GP2078 (Δ[[gene|khtS]]-[[gene|khtT]]-[[gene|khtU]]::''aphA3''), available in [SW|Jörg Stülke]'s lab

BKE09860 (Δ[[gene|khtT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC

BKK09860 (Δ[[gene|khtT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC

pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

27935846

27935846, 31361596, 32603625

14987767, 17679694, 24330391

14987767, 17679694, 24330391, 31061098

[[this]]

binds c-di-AMP, which likely activates export activity [pubmed|31061098]

pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab